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KMID : 0364820100460040359
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Korean Journal of Microbiology
2010 Volume.46 No. 4 p.359 ~ p.365
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Isolation and Taxonomical Characterization of Streptomyces sp. JR-24 with Antibacterial Activity of Bacterial Leaf Spot of Pepper (Xanthomonas axonopodis pv. vesicatoria)
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Han Song-Ih
Lee Hyo-Jin
Whang Kyung-Sook
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Abstract
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Fifty Actinobacteria strains were isolated from rhizosphere soil of Sasa borealis. In the course of screening for antibacterial activity against bacterial leaf spot of pepper (Xanthomonas axonopodis pv. vesicatoria) of isolates, 12 isolates showed strong antibiotic activity. Basis on the 16S rRNA gene sequence, they were belonging to Streptomyces cluster II. Strain JR-24 exhibited strong antibiotic activity against X. axonopodis pv. vesicatoria, had a minimum inhibitory concentration of 10 μl/disc. The strain JR-24 was most closely related to Streptomyces galbus DSM40089T (98.1%), Streptomyces longwoodensis LMG20096T (98%) and Streptomyces capoamus JCM4734T (97.8%). When assayed with the API 20NE and 50 CHE kit, it is positive for utilization of L-arabinose, D-fructose, D-glucose, D-galactose and hydrolysis of gelatin, protein, starch. The strains contained iso-C14:0 (25.93%), iso-C15:0 (10.13%), anteiso-C15:0 (19.29%) and iso-C16:0 (20.35%) as major fatty acids and MK-9 (H4), MK-9 (H6), and MK-9 (H8) as the isoprenoid quinone. Strain JR-24 was suggested new species of genus Streptomyces by nearest neighbors of genotypic relationships and phenotypic characterization. This study was important to microbial resources investigation for environment-friendly agriculture.
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KEYWORD
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antibacterial activity, Streptomyces, Xanthomonas axonopodis pv. vesicatoria
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